Table 2. Cyanobacterial natural product gene clusters detected in Kranji reservoir.

Cluster no.	Pathway type	Pathway name	Reference strain	GI nos.	No. of genes in cluster	No. of genes shared	KS/C domains in pathway	Total KS/C reads	Reference
1	PKS/NRPS	Microcystin	M. aeruginosa PCC7806	6007547–6007556	12	10	4/6	238/249	Tillett et al., 2000
2	PKS	Lectin like	M. aeruginosa M228	7288161–7288163	3	NA	1/0	12/0	Jimbo et al., 2000
3	PKS/NRPS	psm3	M. aeruginosa K-139	150445937–150445949	13	NA	1/4	12/11	Nishizawa et al., 2007
4	PKS/NRPS/FAS	None	M. aeruginosa NIES-843	166365520–166365529	10	1	3/2	857/163	Shulse and Allen, 2011
5	PKS	NIES-843 enediyne	M. aeruginosa NIES-843	166364025	1	1	1/0	168/0	Frangeul et al., 2008
6	PKS	PKS iterative	M. aeruginosa PCC7806	159028726–159028735	10	1	1/0	100/0	Frangeul et al., 2008
7	PKS	PKS1/PKS 3	M. aeruginosa PCC7806	159029973–159029981	9	3	3/0	105/0	Frangeul et al., 2008
8	NRPS	Cyanopeptolin	M. aeruginosa NIES-843	166368741–166368744	4	4	0/6	0/106	Frangeul et al, 2008
9	NRPS	Aeruginosin	M. aeruginosa PCC7806	159030043–159030054	13	10	0/4	0/161	Frangeul et al., 2008
10	NRPS	Micropeptin	M. aeruginosa K-139	223717788–223717791	4	NA	0/7	0/133	Nishizawa et al., 2011
11	NRPS	Oscillapeptin	Planktothrix rubescens NIVA-CYA 98	258380691–258380695	5	NA	0/4	0/24	Blom et al., 2003
12	NRPS	Anabaenopeptin	Planktothrix agardhii NIVA-CYA 126/8	157804708–157804711	4	NA	0/4	0/38	Christiansen et al., 2011
13	PKS/NRPS	Microginin	Planktothrix rubescens NIVA-CYA 98	258380635–258380636	2	NA	1/3	0/6	Rounge et al., 2009
14	NRPS	None	Acaryochloris marina MBIC11017	158339498–158339499	2	NA	0/2	0/2	Swingley et al., 2008
15	NRPS	None	Cyanothece sp. PCC8802	257059705	1	NA	0/3	0/8	Welsh et al., 2008

Note: The newly identified PKS and NRPS gene clusters (MIC2 and MIC3) from recently sequenced Microcystis reference strains (Humbert et al., 2013) were not detected in Kranji Reservoir samples. MIC1 from Humbert et al. (2013) is defined here as Cluster 4.