Bacterial virulence (mean time of death of wax moth larvae after bacterial infection (in h)) between ancestral (A), bacterium-alone (B), bacterium–phage (B+phage), bacterium–protist (B+protist), bacterium–phage–protist (B+phage+protist) and control (M9 buffer) treatments (a and b). (a) Differences between treatment means (five replicates per treatment; mean of eight infected larvae per colony; colony means per replicate, N=5). (b) Differences between different colony type means within treatments (five replicates per treatment; mean of eight infected larvae per colony; means of ancestral-like and small colonies per replicate). Moreover, 60 measurement replicates (larvae) were used for ancestral and control treatments. In both panels, error bars in all panels denote ±1 s.e.m.