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. 2014 Jul 7;15(7):11973–11983. doi: 10.3390/ijms150711973

Figure 4.

Figure 4

LIM domain kinase 1 (Limk1) is a direct target of miR-143 in NSCLC cells. (A) wild type Limk1 3'-untranslated regions (3'-UTR) (WT) or Mutated Limk1 3'-UTR (Mut) of Limk1 sequence; (B) HEK-293 cells were co-transfected with miR-143/miR-NC with WT/Mut 3'-UTR of Limk1. Relative luciferase activity was assayed; and (C) NCI-H23 cells transfected with miR-143 or miR-NC, and Western blot was used to detect the protein level of Limk1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as control. Experiments were performed in triplicate. * p < 0.05, ** p < 0.01 compared with control.