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. 2014 Jul 8;15(7):12119–12134. doi: 10.3390/ijms150712119

Figure 2.

Figure 2

Cordycepin inhibits TNFα production in ex vivo cultured peripheral blood mononuclear cells (PBMCs) of KD (Kawasaki disease) patients. The ex vivo cultured PBMCs of acute KD patients were either left untreated (“C”) or treated with indicated concentrations of cordycepin for 24 h; cell viability was analyzed by MTT assay (A); TNFα in culture supernatant was measured with the ELISA kit (B); The ex vivo cultured PBMCs from healthy controls or the primary BMDMs (bone marrow derived macrophages) were stimulated with LPS (0.1 or 1.0 μg/mL) in the presence or absence of cordycepin (10 μM). TNFα content in culture supernatant was measured with the ELISA kit 24 h after treatment (C,D); and TNFα mRNA expression was examined 6 h after stimulation (E, for BMDMs). The results presented are representative of three independent experiments. The values are expressed as the means ± SD. * p < 0.05 compared with “C” group (A,B); # p < 0.05 (C–E).