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. 2014 Jul 9;15(7):12149–12165. doi: 10.3390/ijms150712149

Figure 5.

Figure 5

HO-1 induction requires Nrf2 accumulation in the nucleus via HO-1 activation. (A) After HepG2 cells were subjected to 20 μM CAPE for 30 min in the presence or absence of U126, ERK inhibitor, Nrf2 in the nucleus, phosphorylated ERK, and HO-1 were determined using western blot analysis; (B) U126, ERK inhibitor prevents the activation of ERK; (C) U126, ERK inhibitor, suppresses Nrf2 accumulation in the nucleus; (D) U126, ERK inhibitor, prevents HO-1 expression; and (E) U126, ERK inhibitor, suppresses ARE promoter activity normalized to vehicle. Sulforaphane (SF) was used as a positive control inducing ARE promoter activity. Data are expressed as mean ± standard deviation of three individual experiments, ## p <0.01, ### p <0.001 vs. vehicle, * p <0.05, ** p < 0.01 vs. CAPE.