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Complete VP2 genes, obtained from the six isolates using primer 3 and primer 4, were confirmed in the recombinant plasmids. Lane M, DNA marker (100 bp); lane N, negative control; lane P, positive control (SCDN strain); lanes 1–6: 1767-bp complete VP2 gene sequences of CPV-YA-ZA1, CPV-YA-ZA2, CPV-YA-ZA4, CPV-YA-ZA5, CPV-LS-ZA1, and CPV-CD-ZA1, respectively, in the recombinant plasmids
