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. 2014 Jul 9;15(7):12166–12187. doi: 10.3390/ijms150712166

Figure 4.

Figure 4

Doubly digested (HindIII and EcoRI) 1767-bp complete VP2 genes inserted into recombinant plasmids. Lane M, DNA marker (250 bp); lanes 1, 3, 5, 7, 9, and 11: recombinant plasmid DNA of CPV-YA-ZA1, CPV-YA-ZA2, CPV-YA-ZA4, CPV-YA-ZA5, CPV-LS-ZA1, and CPV-CD-ZA1, respectively, digested with HindIII and EcoRI; lanes 2, 4, 6, 8, 10, and 12: recombinant plasmid DNA of CPV-YA-ZA1, CPV-YA-ZA2, CPV-YA-ZA4, CPV-YA-ZA5, CPV-LS-ZA1, and CPV-CD-ZA1, respectively digested with HindIII