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. 2014 Jul 17;15(7):12665–12676. doi: 10.3390/ijms150712665

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Akt blockers inhibit TGF-β1-induced GSK3β phosphorylation, β-catenin nuclear translocation and cell proliferation in mouse PSCs. Mouse PSCs were pre-treated with perifosine (2.5 μM) or MK-2206 (5 μM) for one hour, followed by TGF-β1 (25 ng/mL) stimulation. Cells were further cultured for the indicated time point; cytosol and nuclear fractions were isolated, and the expression of the indicated proteins in the corresponding fraction was tested by western blotting (A, B); Cell proliferation was tested by the ³H-thymidine incorporation assay (C). Experiments in this figure were repeated three times, and similar results were obtained. * p < 0.05 “C” stands for the PBS control.