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. 2014 Jul 17;15(7):12665–12676. doi: 10.3390/ijms150712665

Figure 5.

Figure 5

Akt activation and β-catenin are important for TGF-β1-induced cyclin D1/c-myc transcription in mouse PSCs. Mouse PSCs were pre-treated with perifosine (2.5 μM) or MK-2206 (5 μM) for one hour, followed by TGF-β1 (25 ng/mL) stimulation. Cells were further cultured, after 24 h, and the mRNA expression of cyclin D1 (A) and c-myc (C) was tested by real-time PCR. The lentiviral particles containing β-catenin-shRNA-1, β-catenin-shRNA-2 or scramble-shRNA (15 μL/mL each) were added to mouse PSCs (Day 4) for 48 h; afterwards, cells were treated with TGF-β1 (25 ng/mL). Cells were further cultured for 24 h, and the mRNA expression of cyclin D1 (B) and c-myc (D) was tested by real-time PCR. Experiments in this figure were repeated three times, and similar results were obtained. * p < 0.05. “C” stands for the PBS control. Vehicle stands for 0.01% DMSO.