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. 2014 Jul 18;15(7):12778–12790. doi: 10.3390/ijms150712778

Figure 2.

Figure 2

Cordycepin induces GBC-SD cell apoptosis. GBC-SD cells were treated with indicated cordycepin (10–100 μM) for 72 h, cell apoptosis was tested by Annexin V/PI FACS (A–C) and Caspase-3 activity assay (D); Note that for Annexin V FACS assay, the results of five sets of experiments were quantified (B,C); GBC-SD cells were treated with z-VAD-fmk (50 μM) for 1 h, followed by cordycepin (25/100 μM) stimulation, cells were further cultured for 72 h, and cell viability was tested (E); Experiments were repeated five times. * Stands for p < 0.05 vs. control (“C”) group (B–D); # Stands for p < 0.05 vs. vehicle group (E). Vehicle: 0.1% DMSO (E).