Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer design for RT-PCR analysis.
| mRNA | Primers | Annealing Tm (Cycle) |
|---|---|---|
| Wnt5a | Fw: 5'-ACGCTA AGGGTTCCTATGAG-3' | 58 °C (32) |
| Rv: 5'-CATAGCAGCACCAGTGAAAC-3' | ||
| Wnt3a | Fw: 5'-GTTCTGCAGCGAAGTGGTG-3' | 58 °C (32) |
| Rv: 5'-CTGCAGCACGGTATAGGTGA-3' | ||
| β-actin | Fw: 5'-GCTCTCCAGAACATCACTCCTGCC-3' | 58 °C (30) |
| Rv: 5'-CGTTGTCATACCAGGAAATGAGCTT-3' |
Fw, forward; Rv, reverse; Tm, temperature.
