Figure 1.

Effect of DK223 on the migration and proliferation of HaCaT cells. (A) Cells were seeded at adensity of 3 × 10⁴/mL 24 h before the scratch test. Scratch widths were measured 48 h after DK223 treatment. Data represent the mean width percentage ± SD for at least three replicates, * p < 0.05; (B) Cells were seeded at adensity of 3 × 10⁴/mL 24 h before treatment. The impedances were measured and analyzed at 24 h after treatment using the ECIS system software. The line data indicate the migration velocity of cells treated with different concentrations of DK223; (C) Cells were seeded overnight before treatment with varying concentrations of DK223 for 48 h. Cells were then incubated with MTT for 4 h at 37 °C, and the absorbance was measured at 570nm; (D,E) The expression of cell cycle-related proteins in HaCaT cells induced by 2 μM DK223 for 24 h. Protein expression was examined by Western blot analysis with the specific antibodies indicated, and mRNA expression was assessed by RT-PCR with the gene-specific primers indicated. A histogram depicting the ImageJ data analysis resultsis shown. Values represent means ± SD of three independent experiments, * p < 0.05 compared with the control in all under experiments.