Figure 5.

Proliferation and migration of dermal fibroblasts treatedwith DK223 and expression of cell- cycle-related genes in dermal fibroblasts after incubation with DK223. (A) Human dermal fibroblasts were seeded at adensity of 3 × 10⁴/mL for 24 and 48 h before treatment. MTT assay was performed as described previously; (B,C)The expressions of cellcycle-related genes, suchasp21, p53, p27, cyclinE, cyclinD, were examined by Western blot and RT-PCR in 24 h as described previously; (D) Cells were seeded at adensity of 3 × 10⁴/mL 24 h before the scratch test. Scratch widths were measured 48 h after DK223 treatment. Ahistogram shows the results of ImageJ data analysis. Data are represented as the mean distance percentages ±SD from at least three replicates, * p < 0.05 in all under experiments.