Table 1.
Sequences used for genotyping and for RT-PCR
| Gene | Accession number | Primer names | Primer sequences |
|---|---|---|---|
| Leptin receptor transcript variant 2 (Lepr) | NM_010704.2 | 106 | GTCTGATTTGATAGATGGTCTT |
| 65 | AGAATGAAAAAGTTGTTTTGG | ||
| 105 | ACAGGCTTGAGAACATGAACAC | ||
| Villin Cre transgenic construct | Villin-Cre13-Left Villin-Cre13-Right | CAAGCCTGGCTCGACGGCC CGCGAACATCTTCAGGTTCT | |
| Hypocretin (HPRT) | NM_010410.2 | Orexin-Left | TTGGACCACTGCACTGAAGA |
| Orexin-Right | CCCAGGGAACCTTTGTAGAAG | ||
| Proopiomelanocortin-α (POMC) | NM_001278581.1 | POMC-Left | AGTGCCAGGACCTCACCA |
| POMC-Right | CAGCGAGAGGTCGAGTTTG | ||
| CART prepropeptide (CARTPT) | NM_013732.6 | CART-Left | CGAGAAGAAGTACGGCCAAG |
| CART-Right | CTGGCCCCTTTCCTCACT | ||
| Neuropeptide Y (NPY) | NM_023456.2 | NPY-Left | AGAAAACGCCCCCAGAAC |
| NPY-Right | AAGTCGGGAGAACAAGTTTCAT | ||
| Thyrotropin-releasing hormone (TRH) | NM_009426.3 | TRH-Left | AAGACCTCCAGCGTGTGC |
| TRH-Right | CCTCCTTCTCCTCCCTTTTG | ||
| Apolipoprotein A-IV (Apo-AIV) | NM_007468.2 | ApoA4-Left | TGTCCTGGAAGAGGGTACTGA |
| ApoA4-Right | ACCCAGCTAAGCAACAATGC | ||
| Microsomal triglyceride transfer protein (MTP) | NM_001163457.1 | MTP-55-Left | TGCTGGCCAACACGTCTA |
| MTP-55-Right | GCCCAACGTACTTCTAATTTATGG | ||
| Solute carrier family 5 member 1 Slc5a1 (SGLT-1) | NM_019810.4 | SGLT1-Left | GACATCCCAGAGGACTCCAA |
| SGLT1-Right | ACCACTGTCCTCCACAAAGG | ||
| Solute carrier family 2 member 2 Slc2a2 (GLUT2) | NM_031197.2 | GLUT2-Left | CTGCTCTTCTGTCCAGAAAGC |
| GLUT2-Right | TGGTGACATCCTCAGTTCCTC | ||
| Hypoxanthine guanine phosphoribosyl transferase | NM_013556.2 | HRPT1-Left | TGGATACAGGCCAGACTTTGTT |
| (Hprt) | HRPT1-Right | CAGATTCAACTTGCGCTCATC | |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | NM_008084.2 | GAPDH-Left | GTGTCCGTCGTGGATCTG |
| GAPDH-Right | CCTGCTTCACCACCTTCTTG | ||
| TATA box binding protein (TBP) | NM_013684.3 | TBP-Left | CAAACCCAGAATTGTTCTCCT |
| TBP-Right | ATGTGGTCTTCCTGAATCCCT |