Figure 3.

Migration of endothelial cells toward tumor cell conditioned medium. BAEC migration through fibronectin-coated filters in modified Boyden chambers, with the indicated medium in the lower chambers. A) Migration of transfected cells in Boyden chambers was imaged at the indicated times after seeding cells on the tops of the filters (3, 6, and 24 h). The undersides of the filters were fixed and stained with crystal violet solution. B) Staining intensities, indicating densities of migrated cells in duplicate wells from 3 independent experiments. *P < 0.001; **P < 0.003.