Figure 5.

VEGF is sufficient to restore endothelial function in conditioned medium from RLIP76-depleted tumor cells. A) Proliferation of BAECs in conditioned medium from untransfected tumor cells (WT), or tumor cells transfected with RLIP76 shRNA, with or without addition of 5 ng/ml rVEGF assessed at 96 h. rVEGF was added to RLIP76-knockdown cells only. *P < 0.006, **P < 0.01 vs. shRNA without rVEGF. B) BAEC migration (24 h) in modified Boyden chambers. *P < 0.01. C) BAEC cord formation in Matrigel (24 h). *P < 0.01 vs. shRNA without rVEGF. All results shown as means ± sem (n=3).