Figure 1.

GCs stimulate adipogenesis in injured muscle. A) At 5 or 14 d after muscle injury of control or Dex-treated-mice, cryo-cross-sections of TA muscles were immunostained with anti-perilipin (red, top panels) plus either anti-laminin (green, uninjured muscle) or anti-eMyHC (green, injured muscle, middle panels), or stained with Oil-Red-O (bottom panels). Scale bars = 50 μm. B) The number of perilipin⁺ cells per view was counted and averaged (n=5 mice). *P < 0.05 vs. control (Ctrl). C) mRNAs of C/EBPα and PPARγ were evaluated by RT-PCR in injured and uninjured muscles of control or Dex-treated mice. Folds of mRNA expression in injured to uninjured muscles are indicated (n=5 mice). GAPDH was used as internal control. *P < 0.05 vs. control.