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. Author manuscript; available in PMC: 2015 Feb 27.
Published in final edited form as: Cell. 2014 Feb 13;156(5):935–949. doi: 10.1016/j.cell.2014.02.001

Figure 6. sgRNA:target DNA recognition by Cas9.

Figure 6

(A and C–J) Recognition of the guide (A), the guide:target heteroduplex (C), the repeat (D), the anti-repeat (E), the three-way junction (F), stem loop 1 (G), the linker (H), stem loop 2 (I) and stem loop 3 (J). Hydrogen bonds and salt bridges are shown as dashed lines. In (A), the target DNA is omitted, for clarity.

(B) Effects of Cas9 (top) and sgRNA (bottom) mutations on the ability to induce indels (n = 3, error bars show mean ± S.E.M., p values based on unpaired Student’s t-test. N.D., not detectable).