Table 2. Significant gene expression changes (p < 0.05) in chemo-resistant II-45 cells in relation to parental II-45 cells.
| Gene Abbreviation | Functional gene grouping | CisR | PemR | ComboR | GemR | VLBR |
|---|---|---|---|---|---|---|
| Abcb1a* | Drug resistance | 1.6 | -- | -- | -- | 1.9 |
| Abcb1b* | Drug resistance | 1.6 | -- | -- | -- | -- |
| Abcb4 | Drug resistance | -- | −2.2 | -- | −2.6 | 2.8 |
| Abcc2 | Drug resistance | -- | 2.0 | 1.6 | -- | 2.1 |
| Abcg2 | Drug resistance | -- | -- | -- | -- | 2.4 |
| Bcl2 | Drug resistance | -- | -- | 1.3 | -- | -- |
| Bcl2l1 | Drug resistance | -- | 2.0 | -- | -- | -- |
| Rb1 | Drug resistance | -- | -- | −1.8 | -- | -- |
| Crabp1 | Drug metabolism | 6.8 | -- | 2.3 | -- | -- |
| Cyp2b2 | Drug metabolism | -- | -- | 2.2 | -- | 9.5 |
| Cyp2c7 | Drug metabolism | -- | -- | 3.9 | -- | -- |
| Dhfr | Drug metabolism | -- | -- | 1.3 | -- | -- |
| Ephx1 | Drug metabolism | -- | -- | −5.5 | -- | -- |
| Nat2 | Drug metabolism | −1.7 | -- | −1.7 | -- | -- |
| Sod1 | Drug metabolism | 1.3 | -- | 1.2 | -- | -- |
| Ugcg | Drug metabolism | -- | -- | -- | -- | −1.6 |
| Xpa | DNA repair | -- | −1.2 | −1.8 | -- | -- |
| Ccnd1 | Cell cycle | 1.5 | 1.1 | -- | -- | -- |
| Cdkn1a | Cell cycle | -- | -- | −1.8 | -- | -- |
| Fgf2 | Growth factor receptor | -- | -- | −2.3 | -- | -- |
| Ar* | Hormone receptor | −7.7A | −36.8A | −72.1A | 2.8 | -- |
| Ppard | Hormone receptor | −2.3 | -- | -- | -- | -- |
| Pparg | Hormone receptor | -- | -- | −2.4 | −2.1 | -- |
| Gabpa | Transcription factor | 1.8 | -- | -- | -- | 1.7 |
| Myc | Transcription factor | -- | 1.6 | -- | -- | -- |
| Abcb1a | Drug resistance | 1.44 | -- | -- | -- | 1.21 |
| Abcb1b | Drug resistance | 1.40 | -- | -- | -- | -- |
| Ar | Hormone receptor | −7.35 | −33.30 | −49.02 | 3.23 | -- |
*Validated using individual TaqMan Gene Expression Assays; results normalized to Rplp1, Actb and Gapdh.
AAssay reached detection limit therefore the result is likely to be higher than indicated. -- indicates result was not significantly different (p ≥ 0.05). II-45, parental mesothelioma cells; CisR, cisplatin resistant II-45 cells; PemR, pemetrexed resistant II-45 cells; ComboR, combination (cisplatin + pemetrexed) resistant II-45 cells; GemR, gemcitabine resistant II-45 cells; VLBR, vinorelbine resistant II-45 cells. Results generated by rat SABioscience PCR arrays using SYBR green (Qiagen); results normalized to a panel of housekeeping genes (Rplp1, Hprt1, Rpl13a, Ldha, Actb, Rplp1) and are the mean of three biological replicates.