Fig. 7.
Bgn binds to VEGF but does not enhance VEGF-induced signaling. A: The ability of Bgn with a His-tag to bind to increasing doses of VEGF, * compared to 0.1 μg/ml, # compared to 1.0 μ/ml. n = 5 for each experimental variable. The absorbance at 450 nm is a measure of BGN concentration (binding). B: The ability of Bgn core protein to bind to increasing doses of VEGF, * compared to 0.1 μg/ml, # compared to 4.0 μg/ml, † compared to 8.0 μg/ml. n = 3 for each experimental variable. The absorbance at 450 nm is a measure of BGN concentration (binding). One symbol, p < 0.05, two symbols, p < 0.01, three symbols, p b 0.001, four symbols, p b 0.0001. C. VEGF induced signaling is not significantly affected by addition of either Bgn PG or Bgn core protein.