Figure 4.

Representative images of nick end-incorporated nucleotides (for evaluation of apoptosis) immunofluorescent green staining in hares treated with verbascoside loaded liposomal eyedrops. Alkali burn induces in epithelial cell an increasing intensely green nuclear apoptotic staining (a) comparing normal nuclear staining with DAPI (b); merge image shows a plastic picture of this phenomenon (c). Verbascoside treatment shows a large reduction of intensely green apoptotic epithelial cells (d) with respect to nonapoptotic cells stained with DAPI (e); merge image represents clearly this condition (f). Original magnification ×20.