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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Nat Commun. 2014 Jul 15;5:4383. doi: 10.1038/ncomms5383

Fig. 1. N-terminal acetylation of specific factors impacts Sup35 prion aggregate stability, size and associated phenotype.

Fig. 1

a. Lysates from WT (SLL2606), ΔNatA (SY319), and SUP35(S2P) (SY1209) [PSI+] strains were incubated in SDS at the indicated temperatures before separation by SDS-PAGE and immunoblotting for Sup35 The percentage of Sup35 detected at the indicated temperatures relative to 100°C is plotted. Horizontal lines on boxes indicate 25th, 50th, and 75th percentiles; error bars indicate 10th and 90th percentiles (n=6; *p≤ 0.001 by unpaired Student’s t-test in comparison with WT [PSI+] at the same temperature). b. Lysates isolated from WT and ΔNatA [PSI+] strains expressing Sup35 (SLL2606, SY319, respectively) or Sup35S2P (SY1209, SY2154, respectively) were analyzed by semidenaturing detergent agarose gel electrophoresis (SDD-AGE) followed by immunoblotting for Sup35 (samples shown were run in non-contiguous lanes on the same gel). c. Lysates isolated from [PSI+] WT and ΔNatA strains propagating the [PSI+]strong (SLL2606 and SY319, respectively) or [PSI+]weak (SLL2600 and SY1132, respectively) conformation were analyzed by SDD-AGE followed by immunoblotting for Sup35 (samples shown were run in non-contiguous lanes on the same gel). d. 10-fold serial dilutions of cultures of WT ([PSI+] and [psi−]) (SLL2606 and SLL2119), [PSI+] ΔNatA (SY319), [PSI+] SUP35(S2P) (SY1209), [PSI+] SUP35(S2P) ssa1(S2P) (SY1339), [PSI+] SUP35(S2P) ssb1(S2P) (SY1965), [PSI+] SUP35(S2P) ssa1(S2P) ssb1(S2P) (SY2182) expressing strains were grown on rich medium (YPD) and medium lacking adenine (−ade) at 30°C.