Table 1.
Strain Doubling Times
Strain | [PSI] | YPD | Vector | Sup35C |
---|---|---|---|---|
WT | + | 1.7 | 1.9 | 1.9 |
− | 1.7 | 1.9 | 1.9 | |
ΔNatA | + | 2.6 | 3.2 | 3.3 |
− | 2 | 2.4 | 2.5 | |
Δssb1/2 | + | 4 | 4.5 | 4.5 |
− | 3 | 3.4 | 3.3 |
Doubling time (h) in YPD medium for [PSI+] (+) and [psi−] (−) WT (SLL2606 and SLL2119, respectively), ΔNatA (SY319 and SY978, respectively), and Δssb1/2 (SY394 and SY441, respectively) strains. Strains were also transformed with either an empty vector or a plasmid expressing the C-terminal domain of Sup35 (Sup35C, SLL6676), and doubling times were determined in SD–Ura medium.