Table 3.

Differential methylation in cord blood DNA in relation to the combined grandmother’s and mother’s smoking during their pregnancies^a

Chrb	Gene	CpG	Only grandmother smoked in her pregnancy vs. neither smoked			Only mother smoked in her pregnancy vs. neither smoked			Both grandmother and mother smoked in their pregnancies vs. neither smoked
			Coefc	SEd	p-value	Coef	SE	p-value	Coef	SE	p-value
1	GFI1	cg10399789	−0.004	0.004	0.339	−0.042	0.009	5.47E-06	−0.039	0.010	1.14E-04
1	GFI1	cg09662411	−0.001	0.006	0.824	−0.075	0.012	6.89E-10	−0.082	0.013	5.01E-10
1	GFI1	cg06338710	−0.005	0.004	0.260	−0.056	0.010	7.61E-08	−0.065	0.013	6.84E-07
1	GFI1	cg18146737	−0.003	0.007	0.624	−0.104	0.017	4.70E-10	−0.117	0.017	1.50E-11
1	GFI1	cg12876356	−0.006	0.008	0.438	−0.127	0.017	1.45E-14	−0.133	0.019	2.90E-12
1	GFI1	cg18316974	−0.003	0.003	0.444	−0.067	0.010	1.66E-10	−0.070	0.014	2.91E-07
1	GFI1	cg09935388	−0.003	0.008	0.692	−0.137	0.016	1.79E-18	−0.147	0.019	2.48E-14
1	GFI1	cg14179389	0.004	0.006	0.495	−0.083	0.010	3.29E-18	−0.079	0.010	1.45E-16
5	AHRR	cg23067299	−0.003	0.004	0.450	0.025	0.007	2.94E-04	0.025	0.006	6.61E-05
5	AHRR	cg03991871	−0.004	0.002	0.116	−0.021	0.005	2.18E-05	−0.020	0.005	3.71E-05
5	AHRR	cg05575921	−0.002	0.003	0.514	−0.057	0.011	2.67E-07	−0.074	0.009	1.18E-16
5	AHRR	cg21161138	−0.001	0.003	0.777	−0.026	0.005	1.70E-06	−0.027	0.007	1.29E-04
6	HLA-DPB2	cg11715943	−0.007	0.003	0.028	−0.019	0.006	9.76E-04	−0.018	0.005	4.85E-04
7	MYO1G	cg19089201	−0.002	0.002	0.337	0.010	0.003	6.78E-04	0.015	0.003	1.00E-06
7	MYO1G	cg22132788	−0.0002	0.003	0.935	0.021	0.004	2.70E-08	0.023	0.004	4.17E-09
7	MYO1G	cg04180046	0.001	0.004	0.882	0.050	0.009	2.74E-08	0.062	0.008	4.61E-14
7	MYO1G	cg12803068	0.003	0.007	0.685	0.071	0.012	7.89E-09	0.086	0.012	4.63E-13
7	ENSG00000225718	cg04598670	−0.00004	0.006	0.995	−0.038	0.009	4.05E-05	−0.036	0.011	9.81E-04
7	CNTNAP2	cg25949550	−0.001	0.001	0.600	−0.014	0.002	1.31E-17	−0.016	0.002	4.30E-23
8	EXT1	cg03346806	−0.001	0.003	0.732	−0.022	0.005	6.19E-06	−0.007	0.006	1.95E-01
14	TTC7B	cg18655025	−0.002	0.002	0.388	−0.014	0.004	2.83E-04	−0.007	0.004	8.20E-02
15	CYP1A1	cg05549655	0.001	0.004	0.838	0.043	0.008	3.41E-08	0.034	0.009	1.08E-04
15	CYP1A1	cg22549041	−0.007	0.008	0.436	0.075	0.014	1.57E-07	0.057	0.018	1.50E-03
15	CYP1A1	cg11924019	0.001	0.005	0.789	0.043	0.007	4.37E-09	0.040	0.011	1.39E-04
15	CYP1A1	cg18092474	0.001	0.007	0.877	0.068	0.012	4.79E-09	0.059	0.017	4.51E-04
21	RUNX1	cg12477880	−0.002	0.004	0.700	0.026	0.010	1.17E-02	0.054	0.011	1.16E-06

^aN=928 individuals analyzed. “Their pregnancies” indicates the grandmother’s pregnancy with the study mother and the mother’s pregnancy with the study newborn whose cord blood DNA methylation we measured. All analyses adjusted for maternal age, maternal education, and parity.

^bChromosome;

^cRegression coefficient;

^dStandard error for regression coefficient. CpGs with p-values reaching a Bonferroni-corrected statistical significance threshold of 0.05/26=0.0019 are noted in bold.