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Glycosylase activity of Tth UDGb H190 mutants on U-, I-, X-, and O-containing DNA substrates. Cleavage reactions were performed as described under “Experimental Procedures” at 50 °C for 60 min with 100 nm protein and 10 nm substrate. A, Tth UDGb-H190A. B, Tth UDGb-H190N. C, Tth UDGb-H190S.
