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. 2014 May 9;289(26):18110–18120. doi: 10.1074/jbc.M114.555862

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Complementation of the R. etli wreQ mutant strain, CE166. A, silver-stained SDS-PAGE (18% gel) of LPS samples from whole-cell lysates. Lane 1, CE3; lane 2, CE166; lane 3, CE166/pLS31; lane 4, CE166/pJB7; lane 5, CE166/pJB8. LPS I, O-antigen-containing LPS; LPS II, core oligosaccharide-Lipid A lacking O-antigen. B and C, relative content of QuiNAc among the sugars of crude LPS as revealed by GC analysis after conversion of sugars to alditol acetates. CE3, wild-type R. etli strain; CE166, R. etli strain with a Tn5 insertion in wreQ; CE166/pLS31, CE166 strain carrying pLS31 vector, which expresses His₆-WreQ; CE166/pJB7 and CE166/pJB8, CE166 strain carrying pJB7 or pJB8 plasmids, each carrying the wbpV gene. QuiN, quinovosamine.