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. 2014 May 9;289(26):18110–18120. doi: 10.1074/jbc.M114.555862

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FIGURE 3. — Autoradiograms of TLC-separated WbpM-WreQ reaction and control reactions. Lane 1, no enzymes were added to the nucleotide sugar substrate UDP-GlcNAc; lane 2, WbpM reaction for synthesizing the 4-keto intermediate; lane 3, WbpM-WreQ reaction for synthesizing UDP-QuiNAc; lane 4, NADPH in place of NADH; lane 5, negative control, no WbpM; lane 6, negative control, no WreQ; lane 7, no NAD(P)H. NMR analysis confirmed the identities of the spots as follows: Spot I, UDP-GlcNAc; Spot II, 4-keto intermediate; Spot III, UDP-QuiNAc. * indicates NADPH.