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One-dimensional proton NMR spectra obtained from the reaction mixture at different incubation times. A broad resonance at 1.241 ppm is due to methyl groups of the detergent Triton X-100 present in the reaction mixture. This reaction was performed with 1 mm UDP-GlcNAc, 1 mm NADH, 48 μg of WbpM, and 54 μg of WreQ in a total volume of 300 μl. WbpM was added last to start the reaction.
