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. 2014 May 9;289(26):18110–18120. doi: 10.1074/jbc.M114.555862

TABLE 1.

Summary of the proton chemical shifts and multiplet structures for the hexose ring of UDP-d-QuiNAc and UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulose at 30 °C

Standard deviation of the chemical shift values is on the order of 0.004 ppm (as reported by Sparky). Accuracy of J coupling constant estimates is ±0.2 Hz for UDP-d-QuiNAc and ±0.6 Hz for UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulose.

Compound Proton Chemical shift Multiplet structure J coupling constants
ppm Hz
UDP-d-QuiNAc H1 5.422 1:1:1:1 quadruplet 3JH1,H2 = 3.6
3JH1,P = 7.2
H2 3.978 Unresolved overlapped multiplet
H3 3.723 1:2:1 triplet 3JH3,H4 = 9.8
3JH2,H3 = 9.8
H4 3.231 1:2:1 triplet 3JH3,H4 = 9.3
3JH4,H5 = 9.3
H5 3.977 Unresolved overlapped multiplet
H6 1.271 1:1 doublet 3JH6,H5 = 6.0
UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulosea H1 5.433 Poorly resolved multiplet
H2 4.091 Unresolved overlapped multiplet 3JH3,H2 = 10.8
H3 3.803 Doublet
H5 4.091 Unresolved overlapped multiplet
H6 1.218 Doublet 3JH6,H5 = 5.4

a Due to a lower concentration of the latter sample, the spectral quality did not allow confident measurement of the smaller 3J coupling constants at H1 position.