FIGURE 5.

Anisomycin-dependent SIGIRR down-regulation in monocytes and neutrophils. A and B, AM-induced p38 activation. RAW264 (A) and dHL60 (B) cells were stimulated with 100 ng/ml AM for indicated time periods, and the cell lysates were analyzed by Western blotting. C and D, RAW264 (C) and dHL60 (D) cells were treated with indicated concentrations of AM. The SIGIRR mRNA levels were measured by quantitative RT-PCR, and the data were normalized to 18 S rRNA mRNA levels, as an internal control. *, p < 0.05 versus vehicle-treated cells; ANOVA with Dunnett's test (n = 3). E and F, MC (E) and PMN (F) cells were treated with 100 ng/ml AM for 12 h. The SIGIRR mRNA levels were measured by quantitative RT-PCR, and the data were normalized to the mRNA level of 18 S rRNA, as an internal control. *, p < 0.05 versus Vehicle-treated cells; Student's t test (n = 3).