TABLE 3.

Quantification of autopalmitoylation and hydrolysis activities (TLC)

zDHHC9 protein	CoASH	CoASH t0	Palmitate	Palmitoyl-zDHHC9
	pmol/min	pmol	pmol/min	pmol
zDHHA9-GCP16	3.4 (0) ± 0.7	3.7 (0) ± 0.8	3.6 (0) ± 0.6	8.0 (0) ± 1.5
zDHHC9-GCP16	47.8 (44.4) ± 5.6	29.3 (25.6) ± 7.6	44.4 (40.8) ± 12.9	32.7 (24.7) ± 9.6
zDHHC9 R148W-GCP16	231.4 (228.0) ± 20.3	15.7 (12.0) ± 2.1	231.4 (228.0) ± 20.8	20.0 (12.0) ±3.3
zDHHC9 P150S-GCP16	54.1 (50.7) ±7.8	15.7 (12.0) ± 6.6	48.6 (45.0) ± 5.9	20.0 (12.0) ± 3.0