FIGURE 1.

Characterization of purified human FKBP22. A, SDS-PAGE analysis of purified recombinant human FKBP22. Human FKBP22 was purified from an E. coli expression system, and the figure shows the final purified material in the presence and absence of dithiothreitol for lanes 1 and 2, respectively. The purified FKBP22 was run on NuPAGE Novex BisTris 12% gel (Invitrogen) and stained with GelCode Blue Stain Reagent. *, blank lane. B, circular dichroism spectra of FKBPs. The circular dichroism spectrum was measured at 4 °C in 1 mm Tris/HCl, pH 7.5, containing 0.05 mm CaCl₂. The concentration of human FKBP22 (green) was 0.05 mg/ml. The spectra for human FKBP12 (black) and chick FKBP65 (red) are included for comparison.