FIGURE 2.

Interaction of FKBP22 with collagens. Fibril formation of type I (A) and type III (B) collagen in the presence and absence of Hsp47 or FKBP22. A stock solution of type I and type III collagen in 50 mm acetic acid was diluted to a final concentration of 0.1 and 0.2 μm, respectively. The measurements were performed in 0.1 m sodium bicarbonate buffer, pH 7.8, containing 0.15 m NaCl and 1 mm CaCl₂ at 34 °C. Hsp47 was used as a positive control. A, the curves indicate the absence (black) and presence of 0.05 μm Hsp47 (red) and 0.2 μm FKBP22 (green). B, the curves indicate the absence (black) and presence of 0.05 μm Hsp47 (red) and 0.05 μm (green) and 0.1 μm (blue) FKBP22. Shown are thermal melting curves of type I collagen (C) and full-length type III collagen (D) in the presence (blue) and absence (red) of FKBP22. The final protein concentrations were 0.2 and 0.6 μm for collagens and FKBP22, respectively. The melting curves in the presence of FKBP22 are shown after subtraction of the FKBP22-only melting curve.