FIGURE 7.

SEC-MALLS analysis of bezlotoxumab, B1, and bezlotoxumab-B1 immune complexes. A, light scattering and molecular mass distributions of complexes formed at various bezlotoxumab/B1 molar ratios (1:0 (magenta), 0:1 (olive green), 1:5 (bright green), 1:1 (red), and 5:1 (navy blue)) as a function of elution time. B, average detected molecular mass of each complex. The molecular masses of the B1-bezlotoxumab complexes are approximately equal to the sum of B1 alone and bezlotoxumab alone, indicating a 1:1 stoichiometry.