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Alignment of the structures of various CROP-antibody complexes. The backbones of the CROP domains of TcdA and TcdB were used for the alignment. Binding of nanobodies A20.1 (green) and B39 (white) as well as of Fab2 (yellow and cyan) and Fab1 (pink and purple) is shown, as is the TcdA carbohydrate ligand α-Gal-(1,3)-β-Gal-(1,4)-β-GlcNAcO(CH2)8CO2CH3.
