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Evaluation of the self-association of the various FADs in Arabidopsis leaves protoplasts using a RLUC bimolecular complementation assay. A, schematic representation of the orientation of FAD to the LUC (luciferase) fragment with respect to RLUC in which N- and C-terminal LUC fragments are fused to bait or prey genes via a 10-amino acid linker (-GGGGSGGGGS-). B, means and standard errors (n = 4) of relative luminescence units (RLU) of reconstituted RLUC in Arabidopsis protoplast cells.
