FIGURE 4.

Evaluation of the associations of membrane desaturases in Arabidopsis leaf protoplasts with the split luciferase system. A, schematic representation of the plasmid constructs made for expression of genes using RLUC system in which N- and C-terminal Luc (luciferase) fragments are fused to bait or prey genes via a 10-amino acid linker (-GGGGSGGGGS-). B, assay of the activity of reconstituted RLUC by bimolecular complementation in Arabidopsis protoplast cells. Two independent experiments were performed, each consisting of three independent replicates per construct. Luminescence values are expressed as relative luminescence units (RLU). Means and standard deviations of three independent biological replicates per construct within a single representative experiment are presented. Both relative orientations of red fluorescent protein DsRed were included as a negative control.