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C18 fatty acid analysis of PC from yeast cells transformed with Fad2/Fad3
Cells were grown in medium containing 0.1 mm deuterated oleic acid and 0.1 mm 13C-labeled linoleic acids at 30 °C for 4 h followed by 20 h of growth at 18 °C. The values are the means ± S.E. (n = 4).
| 18:1Δ9 | 18:2Δ9,12 | 18:3Δ9,12,15 | |
|---|---|---|---|
| Unlabeled FA | 49.9 ± 0.7 | 26.3 ± 0.1 | 24.7 ± 0.6 |
| 9,10-d2 FA | 50.1 ± 0.7 | 29.1 ± 0.3 | 39.7 ± 0.4 |
| 13C-C1 FA | 0 | 44.6 ± 0.4 | 35.6 ± 0.3 |
