TABLE 1.
FGD6 interactome
Recombinant FGD6 (aa 1–1039) fused to GST was expressed in E. coli. GST-FGD6 bound to glutathione beads was mixed with an osteoclast lysate, and after washing, the bound proteins were identified by MS/MS. In this table the most abundant proteins identified in this scan are seen. GFP-FGD6 was also expressed in osteoclasts and GFP-FGD6 and its bound interactors were immunoprecipitated from osteoclast lysates using anti-GFP antibodies. The eluted proteins were identified by MS/MS. The results from this scan are seen in the right column. See also supplemental Table S1.
| Protein | SwissProt accession no. | Molecular mass | Unique peptides | Sequence coverage | GFP-FGD6 co-IP |
|---|---|---|---|---|---|
| kDa | % | ||||
| GTPases | |||||
| Rac2 | Q05144 | 21.4 | 3 | 24.5 | |
| Cdc42 | P60766 | 21.3 | 12 | 85.9 | |
| GAPs | |||||
| IQGAP1 | Q9JKF1 | 188.8 | 71 | 57.6 | X |
| ARHGAP10 | Q6Y5D8 | 89.4 | 9 | 14.8 | |
| Integrin-binding proteins | |||||
| Talin-1 | P26039 | 269.8 | 57 | 39.6 | |
| Filamin A | Q8BTM8 | 281.2 | 33 | 21.8 | X |
| Actin nucleation factors (WASH complex) | |||||
| F-actin-capping protein subunit α-1 | P47753 | 32.9 | 2 | 16.8 | X |
| F-actin-capping protein subunit α-2 | P47754 | 33.0 | 1 | 10.5 | X |
| F-actin-capping protein subunit β | P47757 | 31.3 | 2 | 12.6 | |
| Actin nucleators (ARP2/3 complex) | |||||
| Actin-related protein 2 | P61161 | 44.8 | 2 | 12.4 | X |
| Actin-related protein 3 | Q99JY9 | 47.4 | 8 | 34.7 | X |
| Actin-related protein 2/3 complex subunit 1B | Q9WV32 | 41.1 | 3 | 11.0 | X |
| Actin-related protein 2/3 complex subunit 3 | Q9JM76 | 20.5 | 2 | 10.1 | X |
| Actin-related protein 2/3 complex subunit 4 | P59999 | 19.7 | 5 | 32.7 | X |
| Coat proteins | |||||
| Vps35 | Q9EQH3 | 91.7 | 4 | 9.8 | X |