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. 2014 May 15;289(26):18360–18372. doi: 10.1074/jbc.M113.541706

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 10. — Extended PY motif constitutes Nedd4-2 dependence in rbNHE3. A, the interaction of Nedd4-2 with hNHE3, rbNHE3, rbNHE3-PY, rbNHE3-ePY, and ratNHE3 was compared by co-immunoprecipitation (IP) in PS120 cells. The interaction of HA-Nedd4-2 was quantified relative to hNHE3. IB, immunoblot. B, ubiquitination of rbNHE3, rbNHE3-PY, and rbNHE3-ePY was determined. The effect of Nedd4-2 knockdown on surface expression (C), and the rates of Na⁺-dependent pH_i recovery of rbNHE3-PY and rbNHE3-ePY were determined (D). E, internalization of rbNHE3 and rbNHE3-ePY is shown. The amounts of internalized NHE3 as percent fraction of surface NHE3 (S) are shown. Data are representative of at least three independent experiments.