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. 2014 May 15;289(26):18360–18372. doi: 10.1074/jbc.M113.541706

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Internalization of hNHE3 is PY motif-dependent. A, PS120/3×FLAG-hNHE3 (left) and PS120/3×HA-rbNHE3 (right) were transfected with shCon (upper panels) or shNedd4-2 (lower panels) and labeled with Alexa Fluor 488-conjugated anti-FLAG or anti-HA antibody without permeabilization. Images of NHE3 (green) and wheat germ agglutinin (WGA; magenta) were captured at time 0 and 3 h of internalization. Arrows point to internalized NHE3. Scale bar, 10 μm. Representative figures from at least three independent experiments are shown. B, PS120 cells expressing 3×FLAG-hNHE3-729 or -710 in PS120 cells were transfected with shCon (top) or shNedd4-2 (bottom) and assayed for internalization as described above.