FIGURE 4.

UDP-Glc activates ERK and STAT3. A, dot blot images from a phosphokinase array on protein samples extracted from cells incubated for 30 min with 100 μm UDP-Glc. The phosphorylated proteins STAT-Tyr⁷⁰⁵ and ERK are encircled. Other spots in array: A3A4, p38a; A7A8, JNK1/2/3; A9A10, GSK-3a/b; B5B6, MSK1/2; B9B10, Akt1/2/3; C1C2, TOR; C3C4, CREB; C7C8, AMPKa2; C9C10, β-catenin; D1D2, Src; D7D8, STAT2; D9D10, STAT5a; E1E2, Fyn; E3E4, Yes; E9E10, STAT5b; F1F2, Hck; F3F4, Chk-2; F5F6, FAK; F7F8, PDGF Rb; F9F10, STAT5a/b (B and C). Western blots of cells incubated for 30–60 min with 100 μm UDP-Glc with antibodies against p-ERK and Tyr(P)⁷⁰⁵-STAT3. Mean ± S.D. of 4 independent experiments are shown. Statistical significance of the difference versus control, **, p = 0.002 (Dunnett's test).