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. 2014 May 20;289(26):18569–18581. doi: 10.1074/jbc.M114.551804

FIGURE 7.

FIGURE 7.

UDP-Glc-induces both Tyr(P)705 and Ser(P)727 phosphorylation of STAT3. A, Western blots of Tyr(P)705-STAT3 and Ser(P)727-STAT3 30–120 min after introduction of 100 μm UDP-Glc. B, quantitation of the changes in phosphorylation of Tyr(P)705 and C, Ser(P)727 of STAT3. Mean ± S.E. from 3 to 6 separate experiments are shown. **, p < 0.01, UDP-Glc versus control, by paired sample t test. D, verification of the effect of STAT3 inhibitor IX and JAK2 inhibitor AG490 on STAT3 Tyr705 phosphorylation after 120 min preincubation with the inhibitors followed by 60 min incubation with UDP-Glc. E, inhibition of the UDP-Glc-induced HAS2 up-regulation by STAT3 inhibitor IX. The cultures were preincubated for 120 min with 50 μm STAT3 inhibitor IX, followed by 120 min in the presence and absence of 100 μm UPD-Glc. Mean ± S.E. of five separate experiments, *, p < 0.05, Dunnett's test.