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Sequence and location of the PCR primers used in the ChIP assays of human HAS2 gene promoter
| Region | Location | Primer sequence (5′to 3′) |
|---|---|---|
| 1 (TSS) | −32 to + 57 | Forward: GGAGGCAGAAGGGCAACAAC |
| Reverse: GTTCAATGGGCTGCTCGAAGC | ||
| 2 | − 481 to − 244 | Forward: GTTACTTAGCTGAAGGGCACC |
| Reverse: GGCCGGTTCTAAACTCCAATG | ||
| 3 | − 1048 to − 655 | Forward: CAGTCATCAGCAGGCTTGTTG |
| Reverse: CTGACGTCAAGTGTCAAAGCC | ||
| 4 | − 1896 to − 1554 | Forward: GGTATTCCCGCATTACGTGTC |
| Reverse: CACTGATTTCCCCCAGCAAC |
