FIGURE 2.

Myo19 truncated constructs. A, schematic diagrams of Myo19 constructs used in this study. To facilitate purification, a FLAG tag was attached to the N terminus of each construct. To facilitate actin-gliding assay, an Avi-tag was attached to the C terminus of the last three constructs. Amino acid numbers of the constructs are indicated. B, SDS-PAGE (4–20%) of purified Myo19-truncated constructs. Myo19-truncated construct was coexpressed with RLC9 and RLC12b in Sf9 cells and purified by anti-FLAG affinity agarose. Lane 1, M19-3IQ; 2, M19-2IQ; 3, M19-1IQ; 4, M19-3IQ-Avi; 5, M19-2IQ-Avi; 6, M19-1IQ-Avi.