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. 2014 May 13;289(26):18535–18548. doi: 10.1074/jbc.M114.569087

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Actin-gliding activity of Myo19. Biotinylated Myo19-truncated constructs were attached to a coverslip via biotinylated BSA and streptavidin, and the movements of rhodamine-labeled actin filaments were recorded with an Olympus IX71 inverted microscope at room temperature (about 25 °C). The velocity was analyzed by MATLAB. The solid line shows a fit to a single Gaussian curve. For details, see ”Experimental Procedures.“ A–C, histogram of multimolecular actin-gliding velocity of Myo19-truncated constructs, i.e. M19-1IQ-Avi (A), M19-2IQ-Avi (B), and M19-3IQ-Avi (C). D and E, direction of the actin filament translocated by M19-1IQ-Avi (D) and Myo5a-ΔT. E, actin filaments were dual fluorescence-labeled. Plus-end was labeled with Alexa 488 (dim) and minus end with rhodamine (bright). Actin filaments were moved toward the minus-end by M19-1IQ-Avi and Myo5a-ΔT.