TABLE 1.
Summary of actin-activated ATPase activity and actin-gliding activity of Myo19 truncated constructs
Actin-activated ATPase activities were measured as described in the legend to Fig. 5 except with the indicated concentration of NaCl. Velocity of actin filaments in actin-gliding activity were measured as described in the legend to Fig 7. All data, except that of Myo5a-1IQ, are the mean ± S.D. of 2–6 independent assays of at least 2 independent preparations.
| Conditions | V0 | Vmax | Kactin | Velocity | |
|---|---|---|---|---|---|
| s−1 | μm | nm/s | |||
| M19-1IQ | 25 mm NaCl | 5.73 ± 0.04 (2) | 10.3 ± 1.3 (2) | 24.5 ± 1.0 (2) | |
| 50 mm NaCl | 0.032 ± 0.001 (3) | 6.75 ± 0.33 (6) | 15.2 ± 4.0 (6) | 27.1 ± 1.2 (2) | |
| 100 mm NaCl | 7.62 ± 0.22 (2) | 24.8 ± 2.3 (2) | 34.5 ± 2.7 (6) | ||
| 150 mm NaCl | 8.77 ± 0.04 (2) | 45.6 ± 5.2 (2) | |||
| M19-2IQ | 50 mm NaCl | 0.035 ± 0.002 (6) | 4.44 ± 0.65 (3) | 17.2 ± 4.9 (3) | 42.4 ± 2.4 (6) |
| M19-3IQ | 50 mm NaCl | 0.035 ± 0.001 (6) | 4.20 ± 0.72 (3) | 20.0 ± 6.8 (3) | 46.8 ± 4.7 (6) |
| Myo5a-1IQ | 50 mm NaCl | 18.21 | 9.60 |