FIGURE 9.

Transcript levels in the colons of DSS-treated WT and Prss31-null B6 mice. A–D, after colitis was induced in WT (n = 5) and Prss31^−/− (n = 5) B6 mice, the two groups of animals were sacrificed, and RNA was isolated to evaluate the levels of the transcripts that encode Cxcl-2 (A), IL-6 (B), Mmp-13 (C), and Cxcl-1 (D). The obtained data were first normalized to those of the GAPDH transcript and then to untreated mice. In this colitis model, the colons of DSS-treated WT mice contained more Cxcl-2 (p = 0.017) and IL-6 (p = 0.055) mRNA than the colons of DSS-treated Prss31^−/− mice. In contrast, the levels of the transcripts that encode Cxcl-1 and MMP-13 were not significantly different between the two groups of DSS-treated animals, in contrast to what occurs in DSS-treated mMCP-6-null mice.