FIGURE 1.

TRPC3 channels contribute to the maintenance of BDNF-induced sustained intracellular Ca²⁺ elevation in rodent microglial cells. A, five representative traces showing the effect of 0.3 μm Pyr3, a selective inhibitor of TRPC3 channels, after the onset of 20 ng/ml BDNF-induced sustained elevation of [Ca²⁺]_i in primary rat microglial cells. Inset, the inset shows a brief (3 min) treatment of BDNF-induced sustained increase in [Ca²⁺]_i in primary rat microglial cells. The average trace of five [Ca²⁺]_i traces in response to BDNF is shown. B, the dose-response effect of different concentrations of Pyr3 on inhibition of the amplitude of [Ca²⁺]_i increase obtained 15 min after BDNF treatment in primary rat microglial cells. Values are the mean ± S.E.