TRPC3 channels contribute to the maintenance of BDNF-induced sustained intracellular Ca2+ elevation in rodent microglial cells.
A, five representative traces showing the effect of 0.3 μm Pyr3, a selective inhibitor of TRPC3 channels, after the onset of 20 ng/ml BDNF-induced sustained elevation of [Ca2+]i in primary rat microglial cells. Inset, the inset shows a brief (3 min) treatment of BDNF-induced sustained increase in [Ca2+]i in primary rat microglial cells. The average trace of five [Ca2+]i traces in response to BDNF is shown. B, the dose-response effect of different concentrations of Pyr3 on inhibition of the amplitude of [Ca2+]i increase obtained 15 min after BDNF treatment in primary rat microglial cells. Values are the mean ± S.E.